At1g14750.2

In the plant Arabidopsis thaliana, At1g14750.2 is a gene model for the locus At1g14750.^[1]

Also known as SDS, this gene model is classified by TAIR as "SDS (SOLO DANCERS)". It is located on chr1, region 5079669-5082418 REVERSE.

Physiological function[]

At1g14750.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g14750.2/Expression

Subcellular localization[]

At1g14750.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g14750.2 has 410 amino-acid residues and a molecular weight Mw = 46.988 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -0.56, or -0.001 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g14750.2/Knockout

Protein interactions[]

At1g14750.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g14750.2 has 2 recognized domains:

Domain_0193_0318 Cyclin_N domain (PF00134, IPR006671) Description: Cyclin, N-terminal;Biological Process: regulation of progression through cell cycle (GO:0000074)

Domain_0320_0410 Cyclin_C domain (PF02984, IPR004367) Description: Cyclin, C-terminal;Biological Process: regulation of progression through cell cycle (GO:0000074), Cellular Component: nucleus (GO:0005634)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g14750.2 MKEIAMRNSK RKPEPTPFAG KKLRSTRLRR KRAQISPVLV QSPLWSKQIG VSAASVDSCS DLLADDNVSC GSSRVEKSSN PKKTLIEEVS DSLDDESSEQ RSEIYSQYSD FDYSDYTPSI FFDSGSEFSE KSSSDSPISH SRSLYLQFKE QFCRSTIPND FGSSCEEEIH SELLRFDDEE VEESYLRLRE RERSHAYMRD CAKAYCSRMD NTGLIPRLRS IMVQWIVKQC SDMGLQQETL FLGVGLLDRF LSKGSFKSER TLILVGIASL TLATRIEENQ PYNSIRKRNF TIQNLRYSRH EVVAMEWLVQ EVLNFKCFTP TIFNFLWFYL KAARANPEVE RKAKSLAVTS LSDQTQLCFW PSTVAAALVV LACIEHNKIS AYQRVIKVHV RTTDNELPEC VKSLDWLLGQ ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-