At1g14810.1

In the plant Arabidopsis thaliana, At1g14810.1 is a gene model for the locus At1g14810.^[1]

This gene model is classified by TAIR as "semialdehyde dehydrogenase family protein". It is located on chr1, region 5102679-5104628 REVERSE.

Physiological function[]

At1g14810.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g14810.1/Expression

Subcellular localization[]

At1g14810.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g14810.1 has 375 amino-acid residues and a molecular weight Mw = 40.743 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -1.55, or -0.004 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g14810.1/Knockout

Protein interactions[]

At1g14810.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g14810.1 has 2 recognized domains:

Domain_0040_0155 Semialdhyde_dh domain (PF01118, IPR000534) Description: Semialdehyde dehydrogenase, NAD - binding;Cellular Component: cytoplasm (GO:0005737), Biological Process: amino acid metabolism (GO:0006520), Molecular Function: oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor (GO:0016620), Molecular Function: NAD binding (GO:0051287)

Domain_0179_0361 Semialdhyde_dhC domain (PF02774, IPR012280) Description: Semialdehyde dehydrogenase, dimerisation region;Cellular Component: cytoplasm (GO:0005737), Biological Process: amino acid biosynthesis (GO:0008652), Molecular Function: oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor (GO:0016620), Molecular Function: protein dimerization activity (GO:0046983)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g14810.1 MATFTHQTPQ THFLSRLPLR AKPRHFSARV KMSLQESAPS LAVVGVTGAV GQEFLSVLSD RDFPYSSIKM LASKRSAGKR VAFDGHEYTV EELTADSFNG VDIALFSAGG SISKEFGPLA AEKGTIVVDN SSAFRMVDGV PLVIPEVNPE AMKGIKVGMG KGALIANPNC STIICLMAVT PLHHHAKVKR MVVSTYQAAS GAGAAAMEEL VQQTREVLEG KPPTCNIFGQ QYAFNLFSHN APILDNGYNE EEMKLVKETR KIWNDTEVKV TATCIRVPVM RAHAESVNLQ FENPLDENTA REILKKAPGV YIIDDRASNT FPTPLDVSNK DDVAVGRIRR DVSQDGNFGL DIFVCGDQIR KGAALNAVQI AEMLL ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-