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In the plant Arabidopsis thaliana, At1g15140.2 is a gene model for the locus At1g15140.[1]

This gene model is classified by TAIR as "oxidoreductase NAD-binding domain-containing protein". It is located on chr1, region 5210637-5212132 REVERSE.

Physiological function[]

At1g15140.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g15140.2/Expression

Subcellular localization[]

At1g15140.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g15140.2 has 271 amino-acid residues and a molecular weight Mw = 29.755 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 2.33, or 0.009 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g15140.2/Knockout

Protein interactions[]

At1g15140.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g15140.2 has 2 recognized domains:

  • Domain_0060_0161 FAD_binding_6 domain (PF00970, IPR008333) Description: Oxidoreductase FAD-binding region;Biological Process: electron transport (GO:0006118), Molecular Function: oxidoreductase activity (GO:0016491)
  • Domain_0174_0257 NAD_binding_1 domain (PF00175, IPR001433) Description: Oxidoreductase FAD/NAD(P)-binding;Biological Process: electron transport (GO:0006118), Molecular Function: oxidoreductase activity (GO:0016491)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g15140.2
MSTLPFAPSV THAHFSHSLS PMFILRHLPL TRHLRLSRNN RVASVVSAAA VRQDASLWTP APLSLIESAA ESLFHISIDI SNAPDLVASY TRPGQYLQIR VPDVEKPSFM AIASPPSLAS SRGAFEFLVK SIAGSTAEIL CGLKKGETVE LSSVMGNGFN IDLIDPPEEY PTVLIFATGS GISPIRSLIE SGFGADRRSD VRLYYGARNL NRMAYQEKFK EWESAGVKVV PVLSQPDDGW KGETGYVQVR LRNVLLSQVC CNSMWIKLIY T
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment[]

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure[]

A predicted structure for this protein is available here.

References[]

  1. This is a test reference.

Further reading[]

General database links[]

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