At1g15140.3

In the plant Arabidopsis thaliana, At1g15140.3 is a gene model for the locus At1g15140.^[1]

This gene model is classified by TAIR as "oxidoreductase NAD-binding domain-containing protein". It is located on chr1, region 5210637-5212132 REVERSE.

Physiological function[]

At1g15140.3/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g15140.3/Expression

Subcellular localization[]

At1g15140.3/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g15140.3 has 271 amino-acid residues and a molecular weight Mw = 29.755 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 2.33, or 0.009 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g15140.3/Knockout

Protein interactions[]

At1g15140.3/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g15140.3 has 2 recognized domains:

Domain_0060_0161 FAD_binding_6 domain (PF00970, IPR008333) Description: Oxidoreductase FAD-binding region;Biological Process: electron transport (GO:0006118), Molecular Function: oxidoreductase activity (GO:0016491)

Domain_0174_0257 NAD_binding_1 domain (PF00175, IPR001433) Description: Oxidoreductase FAD/NAD(P)-binding;Biological Process: electron transport (GO:0006118), Molecular Function: oxidoreductase activity (GO:0016491)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g15140.3 MSTLPFAPSV THAHFSHSLS PMFILRHLPL TRHLRLSRNN RVASVVSAAA VRQDASLWTP APLSLIESAA ESLFHISIDI SNAPDLVASY TRPGQYLQIR VPDVEKPSFM AIASPPSLAS SRGAFEFLVK SIAGSTAEIL CGLKKGETVE LSSVMGNGFN IDLIDPPEEY PTVLIFATGS GISPIRSLIE SGFGADRRSD VRLYYGARNL NRMAYQEKFK EWESAGVKVV PVLSQPDDGW KGETGYVQVR LRNVLLSQVC CNSMWIKLIY T ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-