At1g15210.1

In the plant Arabidopsis thaliana, At1g15210.1 is a gene model for the locus At1g15210.^[1]

Also known as PDR7, and ATPDR7, this gene model is classified by TAIR as "ATPDR7/PDR7 (PLEIOTROPIC DRUG RESISTANCE 7); ATPase, coupled to transmembrane movement of substances". It is located on chr1, region 5231547-5236568 REVERSE.

Physiological function[]

At1g15210.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g15210.1/Expression

Subcellular localization[]

At1g15210.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g15210.1 has 1442 amino-acid residues and a molecular weight Mw = 162.573 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 3.73, or 0.003 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g15210.1/Knockout

Protein interactions[]

At1g15210.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g15210.1 has 5 recognized domains:

Domain_0195_0418 ABC_tran domain (PF00005, IPR003439) Description: ABC transporter related;Molecular Function: ATP binding (GO:0005524), Molecular Function: ATPase activity (GO:0016887)

Domain_0520_0733 ABC2_membrane domain (PF01061, IPR013525) Description: ABC-2 type transporter

Domain_0738_0803 PDR_assoc domain (PF08370, IPR013581) Description: Plant PDR ABC transporter associated

Domain_0878_1068 ABC_tran domain (PF00005, IPR003439) Description: ABC transporter related;Molecular Function: ATP binding (GO:0005524), Molecular Function: ATPase activity (GO:0016887)

Domain_1165_1379 ABC2_membrane domain (PF01061, IPR013525) Description: ABC-2 type transporter_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g15210.1 MDYDPAHAMS RGGSMRQTIS RSVSKASRNM EDIFNTSSRR TKSVNEDEEA LKWASIEKLP TYNRLRTSLM PELGEDDVYG NQILNKAVDV TKLDGEERQK FIDMVFKVAE QDNERILTKL RNRIDRVGIQ LPTVEVRYDH LTVKADCYTG DRSLPSLLNA VRNMGEAALG MIGIRLAKKA QLTILKDVSG IVKPSRMTLL LGPPSSGKTT LLLALAGKLD KSLDVSGEVT YNGYRLNEFV PIKTSAYISQ NDLHVGIMTV KETLDFSARC QGVGTRYDLL NELARREKDA GIFPEADVDL FMKASAAQGV KSSLITDYTL KILGLDICKD TIVGDDMMRG ISGGQKKRVT TGEMIVGPTK TLFMDEISTG LDSSTTFQIV KCLQQIVHLT EATVLISLLQ PAPETFDLFD DIILLSEGQI VYQGPRDHIL EFFESFGFKC PERKGTADFL QEVTSKKDQE QYWVDPNRPY RYIPVSEFAS SFKKFHVGSK LSNELSVPYD KSKSHKAALM FDKYSIKKTE LLKSCWDKEW MLMKRNSFFY VFKTVQIIII AAITSTLYLR TEMHTRNEID ANIYVGSLLF AMIVNMFNGL AEMAMTIQRL PVFYKQRDLL FHPPWTYTLP TFLLGIPISI FESTAWMVVT YYSIGYAPDA ERFFKQFLII FLIQQMAAGI FRFIASTCRT MTIANTGGVL VLLVVFLTGG FLLPRSEIPV WWRWAYWISP LSYAFNAITV NELFAPRWMN KMSGNSTTRL GTSVLNIWDV FDDKNWYWIG VGGLLGFTVI FNGFFTLALT YLDPLGKAQA ILPKEEDEEA KGKAGSNKET EMESVSAKKG MVLPFTPLAM SFDDVKYFVD MPAEMREQGV QETRLQLLKG VTSAFRPGVL TALMGVSGAG KTTLMDVLAG RKTGGYIEGD VRVSGFPKKQ ETFARISGYC EQTDIHSPQV TVRESLIFSA FLRLAKEVSK EDKLMFVDQV MELVELVDLR DAIVGLPGVT GLSTEQRKRL TIAVELVANP SIIFMDEPTS GLDARAAAIV MRAVRNTVDT GRTVVCTIHQ PSIDIFEAFD ELLLMKRGGH VIYSGPLGRN SHKVVEYFES FPGVPKIPEK YNPATWMLEA SSLAAELKLG VDFAELYKAS ALCQRNKALV QELSVPPQGA TDLYFATQFS QNTWGQFKSC LWKQWWTYWR SPDYNLVRFI FTLATSLMIG SVFWQIGGKR SNVQDLTMVI GAIYAAVVFV GINNCSTVQP MVAVERTVFY REKAAGMYSA IPYAISQVTC ELPYVLIQTT YYSLIIYSMV GFEWKASKFL WFIFINYFSF LYWTYYGMMT VSLTPNQQVA SIFASAFYGI FNLFSGFFIP RPKIPKWWVW YYWICPVAWT IYGLITSQYG DVETPIALLG GAPGLTVKQY IKDQYGFESD YMGPVAGVLV GFTVFFAFIF AFCIKTLNFQ SR ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-