In the plant Arabidopsis thaliana, At1g15510.1 is a gene model for the locus At1g15510.[1]
This gene model is classified by TAIR as "pentatricopeptide (PPR) repeat-containing protein". It is located on chr1, region 5329106-5331706 FORWARD.
Physiological function[]
At1g15510.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g15510.1/Expression
Subcellular localization[]
At1g15510.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g15510.1 has 866 amino-acid residues and a molecular weight Mw = 97.701 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 4.53, or 0.005 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g15510.1/Knockout
Protein interactions[]
At1g15510.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g15510.1 has 12 recognized domains:
- Domain_0700_0734 PPR domain (PF01535, IPR002885) Description: Pentatricopeptide repeat_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g15510.1
MASSAQSPHF
YLNPGKSNSF
QSKAYKQRNV
NFYWNFGIRR
LFLRKSQGLS
VLSSSSSSTH
FSNSQLHGLC
ANGKLEEAMK
LLNSMQELRV
AVDEDVFVAL
VRLCEWKRAQ
EEGSKVYSIA
LSSMSSLGVE
LGNAFLAMFV
RFGNLVDAWY
VFGKMSERNL
FSWNVLVGGY
AKQGYFDEAM
CLYHRMLWVG
GVKPDVYTFP
CVLRTCGGIP
DLARGKEVHV
HVVRYGYELD
IDVVNALITM
YVKCGDVKSA
RLLFDRMPRR
DIISWNAMIS
GYFENGMCHE
GLELFFAMRG
LSVDPDLMTL
TSVISACELL
GDRRLGRDIH
AYVITTGFAV
DISVCNSLTQ
MYLNAGSWRE
AEKLFSRMER
KDIVSWTTMI
SGYEYNFLPD
KAIDTYRMMD
QDSVKPDEIT
VAAVLSACAT
LGDLDTGVEL
HKLAIKARLI
SYVIVANNLI
NMYSKCKCID
KALDIFHNIP
RKNVISWTSI
IAGLRLNNRC
FEALIFLRQM
KMTLQPNAIT
LTAALAACAR
IGALMCGKEI
HAHVLRTGVG
LDDFLPNALL
DMYVRCGRMN
TAWSQFNSQK
KDVTSWNILL
TGYSERGQGS
MVVELFDRMV
KSRVRPDEIT
FISLLCGCSK
SQMVRQGLMY
FSKMEDYGVT
PNLKHYACVV
DLLGRAGELQ
EAHKFIQKMP
VTPDPAVWGA
LLNACRIHHK
IDLGELSAQH
IFELDKKSVG
YYILLCNLYA
DCGKWREVAK
VRRMMKENGL
TVDAGCSWVE
VKGKVHAFLS
DDKYHPQTKE
INTVLEGFYE
KMSEVGLTKI
SESSSMDETE
ISRDEIFCGH
SERKAIAFGL
INTVPGMPIW
VTKNLSMCEN
CHDTVKFISK
TVRREISVRD
AEHFHHFKDG
ECSCGD
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.