At1g15520.1

In the plant Arabidopsis thaliana, At1g15520.1 is a gene model for the locus At1g15520.^[1]

Also known as PDR12, and ATPDR12, this gene model is classified by TAIR as "ATPDR12/PDR12 (PLEIOTROPIC DRUG RESISTANCE 12); ATPase, coupled to transmembrane movement of substances". It is located on chr1, region 5331988-5338170 REVERSE.

Physiological function[]

At1g15520.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g15520.1/Expression

Subcellular localization[]

At1g15520.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g15520.1 has 1423 amino-acid residues and a molecular weight Mw = 161.019 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 4.86, or 0.003 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g15520.1/Knockout

Protein interactions[]

At1g15520.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g15520.1 has 5 recognized domains:

Domain_0180_0403 ABC_tran domain (PF00005, IPR003439) Description: ABC transporter related;Molecular Function: ATP binding (GO:0005524), Molecular Function: ATPase activity (GO:0016887)

Domain_0505_0718 ABC2_membrane domain (PF01061, IPR013525) Description: ABC-2 type transporter

Domain_0723_0787 PDR_assoc domain (PF08370, IPR013581) Description: Plant PDR ABC transporter associated

Domain_0863_1053 ABC_tran domain (PF00005, IPR003439) Description: ABC transporter related;Molecular Function: ATP binding (GO:0005524), Molecular Function: ATPase activity (GO:0016887)

Domain_1150_1364 ABC2_membrane domain (PF01061, IPR013525) Description: ABC-2 type transporter_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g15520.1 MEGTSFHQAS NSMRRNSSVW KKDSGREIFS RSSREEDDEE ALRWAALEKL PTFDRLRKGI LTASHAGGPI NEIDIQKLGF QDTKKLLERL IKVGDDEHEK LLWKLKKRID RVGIDLPTIE VRFDHLKVEA EVHVGGRALP TFVNFISNFA DKFLNTLHLV PNRKKKFTIL NDVSGIVKPG RMALLLGPPS SGKTTLLLAL AGKLDQELKQ TGRVTYNGHG MNEFVPQRTA AYIGQNDVHI GEMTVRETFA YAARFQGVGS RYDMLTELAR REKEANIKPD PDIDIFMKAM STAGEKTNVM TDYILKILGL EVCADTMVGD DMLRGISGGQ KKRVTTGEML VGPSRALFMD EISTGLDSST TYQIVNSLRN YVHIFNGTAL ISLLQPAPET FNLFDDIILI AEGEIIYEGP RDHVVEFFET MGFKCPPRKG VADFLQEVTS KKDQMQYWAR RDEPYRFIRV REFAEAFQSF HVGRRIGDEL ALPFDKTKSH PAALTTKKYG VGIKELVKTS FSREYLLMKR NSFVYYFKFG QLLVMAFLTM TLFFRTEMQK KTEVDGSLYT GALFFILMML MFNGMSELSM TIAKLPVFYK QRDLLFYPAW VYSLPPWLLK IPISFMEAAL TTFITYYVIG FDPNVGRLFK QYILLVLMNQ MASALFKMVA ALGRNMIVAN TFGAFAMLVF FALGGVVLSR DDIKKWWIWG YWISPIMYGQ NAILANEFFG HSWSRAVENS SETLGVTFLK SRGFLPHAYW YWIGTGALLG FVVLFNFGFT LALTFLNSLG KPQAVIAEEP ASDETELQSA RSEGVVEAGA NKKRGMVLPF EPHSITFDNV VYSVDMPQEM IEQGTQEDRL VLLKGVNGAF RPGVLTALMG VSGAGKTTLM DVLAGRKTGG YIDGNITISG YPKNQQTFAR ISGYCEQTDI HSPHVTVYES LVYSAWLRLP KEVDKNKRKI FIEEVMELVE LTPLRQALVG LPGESGLSTE QRKRLTIAVE LVANPSIIFM DEPTSGLDAR AAAIVMRTVR NTVDTGRTVV CTIHQPSIDI FEAFDELFLL KRGGEEIYVG PLGHESTHLI NYFESIQGIN KITEGYNPAT WMLEVSTTSQ EAALGVDFAQ VYKNSELYKR NKELIKELSQ PAPGSKDLYF PTQYSQSFLT QCMASLWKQH WSYWRNPPYT AVRFLFTIGI ALMFGTMFWD LGGKTKTRQD LSNAMGSMYT AVLFLGLQNA ASVQPVVNVE RTVFYREQAA GMYSAMPYAF AQVFIEIPYV LVQAIVYGLI VYAMIGFEWT AVKFFWYLFF MYGSFLTFTF YGMMAVAMTP NHHIASVVSS AFYGIWNLFS GFLIPRPSMP VWWEWYYWLC PVAWTLYGLI ASQFGDITEP MADSNMSVKQ FIREFYGYRE GFLGVVAAMN VIFPLLFAVI FAIGIKSFNF QKR ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-