At1g15700.1

In the plant Arabidopsis thaliana, At1g15700.1 is a gene model for the locus At1g15700.^[1]

Also known as ATPC2, this gene model is classified by TAIR as "ATPC2 (ATP synthase gamma chain 2)". It is located on chr1, region 5402624-5403784 REVERSE.

Physiological function[]

At1g15700.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g15700.1/Expression

Subcellular localization[]

At1g15700.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g15700.1 has 386 amino-acid residues and a molecular weight Mw = 42.679 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -0.45, or -0.001 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g15700.1/Knockout

Protein interactions[]

At1g15700.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g15700.1 has 1 recognized domains:

Domain_0061_0383 ATP-synt domain (PF00231, IPR000131) Description: ATPase, F1 complex, gamma subunit;Biological Process: ATP synthesis coupled proton transport (GO:0015986), Cellular Component: membrane (GO:0016020), Cellular Component: proton-transporting two-sector ATPase complex (GO:0016469), Molecular Function: hydrogen-transporting ATP synthase activity, rotational mechanism (GO:0046933), Molecular Function: hydrogen-transporting ATPase activity, rotational mechanism (GO:0046961)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g15700.1 MTGSISTSWL LSSPSNSNSA SSSESYSFIA TLKPVRYYPF QSLTPNRISS RSPLPSIQIR AGIRELRERI DSVKNTQKIT EAMRLVAAAR VRRAQDAVIK GRPFTETLVE ILYSINQSAQ LEDIDFPLSI VRPVKRVALV VVTGDKGLCG GFNNAVTKKA TLRVQELKQR GIDCVVISVG KKGNAYFSRR DEFDVDKCIE GGGVFPTTKE AQVIADDVFS LFVSEEVDKV ELVYTKFVSL VKSDPVIHTL LPLSMKGESC DVKGECVDAI EDEMFRLTSK DGKLAVERTK LEVEKPEISP LMQFEQDPVQ ILDAMMPLYL NSQILRALQE SLASELASRM NAMSNATDNA VELKKNLTMA YNRARQAKIT GELLEIVAGA EALRES ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-