At1g16445.1

In the plant Arabidopsis thaliana, At1g16445.1 is a gene model for the locus At1g16445.^[1]

This gene model is classified by TAIR as "methylase-related". It is located on chr1, region 5617805-5619297 FORWARD.

Physiological function[]

At1g16445.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g16445.1/Expression

Subcellular localization[]

At1g16445.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g16445.1 has 274 amino-acid residues and a molecular weight Mw = 30.160 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 4.66, or 0.017 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g16445.1/Knockout

Protein interactions[]

At1g16445.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g16445.1 has 1 recognized domains:

Domain_0129_0272 rRNA_methylase domain (PF06962, IPR010719) Description: Putative rRNA methylase_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g16445.1 MAAGFFQAEM SILSSTLARS YSLPIRKTLM TFDFRIAMQR NPCLRIRRSC VAAFSSTPSH SRNFPIRGLE DVFVGYLFGR KKATEVAHVV WEQVIQKGDT VIDATCGNGN DTLAMLKMVM HDSVGCGGYV YAMDIQKDAI ESTSSLLDQA VGSKEKECVK LFNLCHSKMG EIVPENARVR MVAFNLGYLP GGNKSIITLS DTTLSALKAA ERILKPGGLI SLVVYIGHPG GREELEVVEA FGSGLPVSDW ICCKFQMLNR PLAPVLVFMF KREN ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-