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In the plant Arabidopsis thaliana, At1g17590.2 is a gene model for the locus At1g17590.[1]

This gene model is classified by TAIR as "CCAAT-binding transcription factor (CBF-B/NF-YA) family protein". It is located on chr1, region 6050474-6051977 REVERSE.

Physiological function[]

At1g17590.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g17590.2/Expression

Subcellular localization[]

At1g17590.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g17590.2 has 328 amino-acid residues and a molecular weight Mw = 36.469 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 8.33, or 0.025 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g17590.2/Knockout

Protein interactions[]

At1g17590.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g17590.2 has 1 recognized domains:

  • Domain_0171_0227 CBFB_NFYA domain (PF02045, IPR001289) Description: CCAAT-binding transcription factor, subunit B;Molecular Function: transcription factor activity (GO:0003700), Cellular Component: nucleus (GO:0005634), Biological Process: regulation of transcription, DNA-dependent (GO:0006355)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g17590.2
MDKKVSFTSS VAHSTPPYLS TSISWGLPTK SNGVTESLSL KVVDARPERL INTKNISFQD QDSSSTLSSA QSSNDVTSSG DDNPSRQISF LAHSDVCKGF EETQRKRFAI KSGSSTAGIA DIHSSPSKAN FSFHYADPHF GGLMPAAYLP QATIWNPQMT RVPLPFDLIE NEPVFVNAKQ FHAIMRRRQQ RAKLEAQNKL IKARKPYLHE SRHVHALKRP RGSGGRFLNT KKLQESTDPK QDMPIQQQHA TGNMSRFVLY QLQNSNDCDC STTSRSDITS ASDSVNLFGH SEFLISDCPS QTNPTMYVHG QSNDMHGGRN THHFSVHI
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment[]

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure[]

A predicted structure for this protein is available here.

References[]

  1. This is a test reference.

Further reading[]

General database links[]

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