At1g20310.1

In the plant Arabidopsis thaliana, At1g20310.1 is a gene model for the locus At1g20310.^[1]

This gene model is classified by TAIR as "similar to unknown protein [Arabidopsis thaliana] (TAIR:AT1G76070.1); similar to syringolide-induced protein 14-1-1 [Glycine max] (GB:BAB86891.1)". It is located on chr1, region 7032298-7033020 FORWARD.

Physiological function[]

At1g20310.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g20310.1/Expression

Subcellular localization[]

At1g20310.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g20310.1 has 240 amino-acid residues and a molecular weight Mw = 26.610 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 23.88, or 0.100 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g20310.1/Knockout

Protein interactions[]

At1g20310.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g20310.1 has no known domains._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g20310.1 MEKASNSNKS TKFSKMLQRA MSIGHSAAPF SPRRDFHQHR TTSTANRGIF FSSPLVPTAA RVRRNTKYEA VFAEPTSPKV SCIGQVKLAR PKCPEKKNKA PKNLKTASSL SSCVIKEEDN GSFSKLKRIF SMRSYPSRKS NSTAFAAAAA REHPIAEVDA VTAAPSLGAM KKFASSREAL GGFDWTVQMK REKEDVMIPC SVGIPLTQLE DLSLCPKPKS EVNLWKRRTM DRPKPLQVIK ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-