At1g25112.1

In the plant Arabidopsis thaliana, At1g25112.1 is a gene model for the locus At1g25112.^[1]

This gene model is classified by TAIR as "similar to unknown protein [Arabidopsis thaliana] (TAIR:AT1G25025.1); similar to unknown protein [Arabidopsis thaliana] (TAIR:AT1G25180.1); similar to PREDICTED: hypothetical protein [Homo sapiens] (GB:XP_001129546.1)". It is located on chr1, region 8815173-8815949 FORWARD.

Physiological function[]

At1g25112.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g25112.1/Expression

Subcellular localization[]

At1g25112.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g25112.1 has 258 amino-acid residues and a molecular weight Mw = 27.725 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 35.54, or 0.138 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g25112.1/Knockout

Protein interactions[]

At1g25112.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g25112.1 has no known domains._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g25112.1 MSCQRGIPPD TQRHRAARQL TRPKYPASRS VSSSSSAHPS QAARQLSPPK YPASRSVSSS SSAHPSQAAR QLSPPKYPAS RSVSNSSSAH PSQAARQLTR PKYPASRSVS SSSSAHPSQA DRQLTRLKQL VSSPLRSIPP AGPSQAARQL TRLNQLVSSP LRSIPPAGPS QTARQLTRPK YPASRFVSSS SSAHPSQAAR QLTRLKQLVS SPVSSSSSAH PYQAARQLTR PKYLVCPSIL SNSFTRPSEV SLRPARPK ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-