In the plant Arabidopsis thaliana, At1g25220.1 is a gene model for the locus At1g25220.[1]
Also known as TRP4, and ASB1, this gene model is classified by TAIR as "ASB1 (ANTHRANILATE SYNTHASE BETA SUBUNIT 1); anthranilate synthase". It is located on chr1, region 8837417-8839465 REVERSE.
Physiological function[]
At1g25220.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g25220.1/Expression
Subcellular localization[]
At1g25220.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g25220.1 has 276 amino-acid residues and a molecular weight Mw = 30.461 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 1.33, or 0.005 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g25220.1/Knockout
Protein interactions[]
At1g25220.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g25220.1 has 1 recognized domains:
- Domain_0076_0266 GATase domain (PF00117, IPR000991) Description: Glutamine amidotransferase class-I;Molecular Function: catalytic activity (GO:0003824)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g25220.1
MAASTLYKSC
LLQPKSGSTT
RRLNPSLVNP
LTNPTRVSVL
GKSRRDVFAK
ASIEMAESNS
IPSVVVNSSK
QHGPIIVIDN
YDSFTYNLCQ
YMGELGCHFE
VYRNDELTVE
ELKKKNPRGV
LISPGPGTPQ
DSGISLQTVL
ELGPLVPLFG
VCMGLQCIGE
AFGGKIVRSP
FGVMHGKSSM
VHYDEKGEEG
LFSGLSNPFI
VGRYHSLVIE
KDTFPSDELE
VTAWTEDGLV
MAARHRKYKH
IQGVQFHPES
IITTEGKTIV
RNFIKIVEKK
ESEKLT
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.