At1g27385.1

In the plant Arabidopsis thaliana, At1g27385.1 is a gene model for the locus At1g27385.^[1]

This gene model is classified by TAIR as "similar to unnamed protein product [Ostreococcus tauri] (GB:CAL52738.1); similar to Os02g0507400 [Oryza sativa (japonica cultivar-group)] (GB:NP_001046922.1); similar to OSIGBa0148P16.4 [Oryza sativa (indica cultivar-group)] (GB:CAH66570.1); contains InterPro domain Protein of unknown function DUF493; (InterPro:IPR007454)". It is located on chr1, region 9511116-9512807 REVERSE.

Physiological function[]

At1g27385.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g27385.1/Expression

Subcellular localization[]

At1g27385.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g27385.1 has 202 amino-acid residues and a molecular weight Mw = 22.060 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 2.77, or 0.014 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g27385.1/Knockout

Protein interactions[]

At1g27385.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g27385.1 has 1 recognized domains:

Domain_0111_0202 DUF493 domain (PF04359, IPR007454) Description: Protein of unknown function DUF493_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g27385.1 MACKTILRSV FVSESRRTSG ARRCFFLPPS PASVPVHGLF PAPKSLCFNG FASVPERATR LNCSHNDQSD QGPPQEAVLK AISEVSKTDG RVGKTTNMII GGTVADDSAK DWLELDQKVN TYPTERGFTA IGTGGNDFVH AMVVAVESVI DRQIPEDCVK QTLSSKGKYV SVNIGPIQVV SSEQVQAVYN AMRRDERMKY FL ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-