At1g27510.1

In the plant Arabidopsis thaliana, At1g27510.1 is a gene model for the locus At1g27510.^[1]

This gene model is classified by TAIR as "similar to EX1 (EXECUTER1) [Arabidopsis thaliana] (TAIR:AT4G33630.2); similar to unknown protein [Oryza sativa (japonica cultivar-group)] (GB:BAD44852.1)". It is located on chr1, region 9554932-9558280 REVERSE.

Physiological function[]

At1g27510.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g27510.1/Expression

Subcellular localization[]

At1g27510.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g27510.1 has 651 amino-acid residues and a molecular weight Mw = 72.098 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -31.09, or -0.048 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g27510.1/Knockout

Protein interactions[]

At1g27510.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g27510.1 has no known domains._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g27510.1 MATTQPCLIG QIIAVPQFHI LFSPRNSLKP ELSTNKRTNF SVSIGLRHSF ASSISTCNPK APSLSCLRNC AAVDGADTSS SEDKWDWDWD RWNRHFSEIE EVESVVSLLK SQLEDAVEKE DFEEAVKLKQ AISEATVDDA VAEIMRQLQT AVNEERYHDA SRLCNETGSG LVGWWVGLPR DSEEPFGRIV HITPGVGRFI GKSYSPRQLV AEAAGTPLFE IFVIKDTDGG YVMQVVYVQH VKQNLTISEN SFSKVQQSSK SSINDPSILD VRGSELKVDK KEDTQLNAGE PTEEGIKNVI KFLKDKIPGL KLKVMDVIKI PEEEIVGSDD ATEELVGEGT EETNSSDDEE EVEEEENDSI EAISSMDSAD YGKHSNTKLV IGGVLHNIED SSIDDEIVRV SANIMDTERD SFILHVPGRS KRDIDTRKNR VSKEQVTALA AQGLSDLLPP EVAEAFWGEK ASLKVSKHVH EIVKLAINQA QKGNHLSEYT AFNRIITPES NLDPFDGLYV GAFGPYGTEI VQLKRKYGRW DDAEGSNSSD IEFFEYVEAV KLTGDPNVPA GQVTFRARIG NGSRMTNHGL FPEELGVLAS YRGQGKIADF GFKKPRWVEG KLLKLNGKGM GPYVKGADLG FLYIGPEQSF LVLFNRLRLP E ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-