At1g29410.2

In the plant Arabidopsis thaliana, At1g29410.2 is a gene model for the locus At1g29410.^[1]

Also known as PAI3, this gene model is classified by TAIR as "PAI3 (PHOSPHORIBOSYLANTHRANILATE ISOMERASE 3); phosphoribosylanthranilate isomerase". It is located on chr1, region 10298691-10299916 REVERSE.

Physiological function[]

At1g29410.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g29410.2/Expression

Subcellular localization[]

At1g29410.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g29410.2 has 244 amino-acid residues and a molecular weight Mw = 26.524 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -0.67, or -0.003 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g29410.2/Knockout

Protein interactions[]

At1g29410.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g29410.2 has 1 recognized domains:

Domain_0068_0243 PRAI domain (PF00697, IPR001240) Description: N-(5'phosphoribosyl)anthranilate isomerase (PRAI);Molecular Function: phosphoribosylanthranilate isomerase activity (GO:0004640), Biological Process: tryptophan metabolism (GO:0006568)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g29410.2 MSTGISSDLH LHPRALNFSK TSKSGLSNRK VSFSSVGYAQ NRKLSCSVSS TENVAPKDDD RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR SISLSVAKDI SQVAREGGAK PVGVFVEDDE NTILRAADSS DLELVQLHGN SSRAAFSRLV RERKVIYVLN ANEDGKLLNV VPEEDGHLAD WILVDSATGG RYLDQLLSFF ALSHCNVFLR GTSYTITLVH ETVCLSQVTE ISRV ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-