In the plant Arabidopsis thaliana, At1g31150.1 is a gene model for the locus At1g31150.[1]
At1g31150.1/Header
Physiological function[]
At1g31150.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g31150.1/Expression
Subcellular localization[]
At1g31150.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At1g31150.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g31150.1/Knockout
Protein interactions[]
At1g31150.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At1g31150.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g31150.1
MNQIKDPNPN
LPERLFAVDC
YPDKARLNIY
SRPEYLGTIA
NLLKGSEEWE
RIKSSQFGKL
FEFPVARCSH
SGKLIHGLLS
RQVVTKKKHE
LWFVFGGHPI
RFSIREFHIV
TGLRCGKLPT
EDEVKKHQDS
KYLSVWNRLF
GEKRMVTIGD
VLEMLQKKKL
SSWKKLCLAL
IVIVDGVVVC
NDQSFVTLDF
VEMLNDIDFF
LEYPWGRKAF
LATIRRFGPP
KDAPNPLGKL
KKRLKQKTSA
CYGFPLALQL
QVFESIPVIL
ERIKDPNDLR
DFTERSSEGL
SIAFLLRKSY
ILDAEVDDKL
VVAHTLEPKG
MINESELVWE
TEVSDPKVDY
ILRLINEGHC
FKPFEWPHLK
AANQEVNIPD
IDGDSLEDVT
DVSQARPKKP
LTRSSKKEKE
VVKPPSIHKH
TSQEKIKAKV
HVGGESNDPG
YVTLAYLNDM
KEWILKQNQD
LRNKIREDVK
EIVVGPKSPA
GGASNLRQSN
RLNTNSKGKT
DASPPKKTGN
TETRKRKRKQ
VEVHSLSSGS
ESAGDIEGEE
DVQSTWKEDG
NAPSSGTFDE
CDDYGGALKI
TQEGDVMQEE
SDFDHCSSNC
TKMKESMMRE
IENLELKLQL
YGGHGLNNLT
YDELLHFEHQ
LESSLHIARA
HKQEQQQQQT
DKLKENLMVC
QRHEDDDIMH
RQVKKENPPA
FFSSSRRHNQ
PQD
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.