At1g31280.1

In the plant Arabidopsis thaliana, At1g31280.1 is a gene model for the locus At1g31280.^[1]

At1g31280.1/Header

Physiological function[]

At1g31280.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g31280.1/Expression

Subcellular localization[]

At1g31280.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

At1g31280.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g31280.1/Knockout

Protein interactions[]

At1g31280.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

At1g31280.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g31280.1 MERGGYRGGR GDGRGRGGRG YGGGGGGGEQ GRDRGYGGGE QGRGRGSERG GGNRGQGRGE QQDFRSQSQR GPPPGHGGRG TTQFQQPRPQ VAPQPSQAPA SYAGSVGGVA GRGAWGRKPQ VPSDSASPST STTVVSEPVR VAEVMNLKPS VQVATSDRKE PMKRPDRGGV VAVRRVNLYV NHYKVNFNPE SVIRHYDVEI KGEIPTKKVS RFELAMVRDK VFTDNPDEFP LAMTAYDGQK NIFSAVELPT GSYKVEYPKT EEMRGRSYTF TIKQVNVLKL GDLKEYMTGR SSFNPRDVLQ GMDVVMKEHP SKCMITVGKS FFTRETEPDE DFRFGVIAAK GYRHTLKPTA QGLSLCLDYS VLAFRKAMSV IEYLKLYFNW SDMRQFRRRD VEEELIGLKV TVNHRKNKQK LTIVGLSMQN TKDIKFDLID QEGNEPPRKT SIVEYFRIKY GRHIVHKDIP CLDLGKNGRQ NFVPMEFCDL VEGQIYPKDN LDKDSALWLK KLSLVNPQQR QRNIDKMIKA RNGPSGGEII GNFGLKVDTN MTPVEGRVLK APSLKLAERG RVVREEPNPR QNNQWNLMKK GVTRGSIVKH WAVLDFTASE RFNKMPNDFV DNLIDRCWRL GMQMEAPIVY KTSRMETLSN GNAIEELLRS VIDEASRKHG GARPTLVLCA MSRKDDGYKT LKWIAETKLG LVTQCFLTGP ATKGGDQYRA NLALKMNAKV GGSNVELMDT FSFFKKEDEV MFIGADVNHP AARDKMSPSI VAVVGTLNWP EANRYAARVI AQPHRKEEIQ GFGDACLELV KAHVQATGKR PNKIVIFRDG VSDAQFDMVL NVELLDVKLT FEKNGYNPKI TVIVAQKRHQ TRFFPATNND GSDKGNVPSG TVVDTKVIHP YEYDFYLCSH HGGIGTSKPT HYYTLWDELG FTSDQVQKLI FEMCFTFTRC TKPVSLVPPV YYADMVAFRG RMYHEASSRE KNFKQPRGAS TSAASLASSL SSLTIEDKAI FKLHAELENV MFFV ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-