At1g31290.1

In the plant Arabidopsis thaliana, At1g31290.1 is a gene model for the locus At1g31290.

At1g31290.1/Header

Physiological function

At1g31290.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression

At1g31290.1/Expression

Subcellular localization

At1g31290.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties

At1g31290.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype

At1g31290.1/Knockout

Protein interactions

At1g31290.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure

At1g31290.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence

>At1g31290.1
MDRGGYRGGR GDGRGRGGGG DRGRGYSGRG DGRGRGGDGD RGYSGRGDGH GRGGGGDRGR GYSGRGDGRG RGGGGDRGRG YSGRGDGHGR GGGGDRGRGY SGRGRGFVQD RDGGWVNPGQ SSGGHVRGRG TQLQQPPPQE VPPSSSQAQV SQGVAPGDVG QGGVGDVGRD GVGDVGRDGV GDVGQGGVGD VGQVGVGDVG QGGVGDVGQG GVGDVGRDGV GDVGRDGVGD VGRGGVGDRG QSQSGLSSGH FGRGTQLQQP QPQAVSQSSS QGQVSQSFAT GGVGLGAWAR KPQLFSDSTV LPSSSSSNVV ASHTASGSQV MTPKPSSSDK KEPVKRPDKG GNIKVKGVIN LSVNHFRVSF STESVIRHYD VDIKGENSSK KISRFELAMV KEKLFKDNND FPNAMTAYDG QKNIFSAVEL PTGSFKVDFS ETEEIMRGRS YTFIIKQVKE LKLLDLQAYI DGRSTFIPRD VLQGMDVVMK EHPSKRMITV GKRFFSTRLE IDFGYGVGAA KGFHHTLKPT VQGLSLCLNS SLLAFRKAIS VIEYLKLYFG WRNIRQFKNC RPDDVVQELI GLKVTVDHRK TKQKFIIMGL SKDDTKDIKF DFIDHAGNQP PRKISIVEYF KEKYGRDIDH KDIPCLNLGK KGRENFVPME FCNLVEGQIF PKEKLYRDSA AWLKELSLVT PQQRLENINK MIKSSDGPRG GDIIGNFGLR VDPNMTTVEG RVLEAPTLKL TDRRGNPIHE KLMSESNQWN LTTKGVTKGS IIKHWAVLDF TASESLKKKM PGYFVNKLIE RCKGLGMQME APIVCKTSSM ETLYDGNALE ELLRSVIDEA SHNHGGACPT LVLCAMTGKH DGYKTLKWIA ETKLGLVTQC FLTISAIKGE TVSDQYLANL ALKINAKVGG TNVELVDNIF SFFKKEDKVM FIGADVNHPA AHDNMSPSIV AVVGTLNWPE ANRYAARVKA QSHRKEEIQG FGETCWELIE AHSQAPEKRP NKIVIFRDGV SDGQFDMVLN VELQNVKDVF AKVGYNPQIT VIVAQKRHQT RFFPATTSKD GRAKGNVPSG TVVDTTIIHP FEYDFYLCSQ HGAIGTSKPT HYYVLSDEIG FNSNQIQKLI FDLCFTFTRC TKPVALVPPV SYADKAASRG RVYYEASLMK KNSKQSRGAS SSSASVASSS SSVTMEDKEI FKVHAGIENF MFFV ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure

A predicted structure for this protein is available here.

Further reading

• PubMed references for At1g31290

• PubMed Central references for At1g31290

• Google Scholar references for At1g31290

General database links

• TAIR Locus At1g31290

• TIGR page for At1g31290

• KEGG page for At1g31290

• SWISS-PROT entry for At1g31290

• Structural model of At1g31290

• NCBI structural data for At1g31290

• NCBI Entrez for At1g31290

• Gramene page for At1g31290

• MIPS MAtDB gene page for At1g31290

• MIPS SESAM alignment for At1g31290

• MIPS PENDANT general properties

• MIPS PENDANT general sequence alignment

• NCBI gene data for At1g31290

• NCBI protein data for At1g31290

• NCBI location on chromosome

• SUBA subcellular localization

• Arabidopsis MPSS Plus on At1g31290