In the plant Arabidopsis thaliana, At1g31500.1 is a gene model for the locus At1g31500.[1]
This gene model is classified by TAIR as "endonuclease/exonuclease/phosphatase family protein". It is located on chr1, region 11273802-11276515 REVERSE.
Physiological function[]
At1g31500.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g31500.1/Expression
Subcellular localization[]
At1g31500.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g31500.1 has 388 amino-acid residues and a molecular weight Mw = 43.846 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is -2.90, or -0.007 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g31500.1/Knockout
Protein interactions[]
At1g31500.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g31500.1 has 1 recognized domains:
- Domain_0063_0383 Exo_endo_phos domain (PF03372, IPR005135) Description: Endonuclease/exonuclease/phosphatase_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g31500.1
MFSSTTLHHL
PRPNLLLPSS
RVCRKVISRR
MSTNPAIEPK
VRKFESVEGV
DIGSRNKSDG
IRFRLVSYNI
LAQVYVKSAL
LPHSPPACLK
WKARSHAILS
VLKNLQADFF
CLQEVDEYDS
FYRNNMDSLG
YSGIYIQRTG
QRKRDGCAIF
YKPSCAELVT
KERIEYNDLV
DSIKADSVSC
SEQKIETSNE
GKDSRKDSRD
LNDPLVRLKR
DCVGIMAAFR
INKPFQHIVI
VANTHLYWDP
ELADVKLAQA
KYLLSRLAQF
KTLISDEFEC
TPSLLLAGDF
NSIPGDMVYS
YLVSGNAKPT
ETIEEEEAPV
PLSSVYEVTR
GEPKFTNCTP
GFTNTLDYIF
ISPSDFIKPV
SILQLPEPDS
PDVVGFLPNH
HHPSDHLPIG
AEFEIRRE
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.