At1g31500.3

In the plant Arabidopsis thaliana, At1g31500.3 is a gene model for the locus At1g31500.^[1]

This gene model is classified by TAIR as "endonuclease/exonuclease/phosphatase family protein". It is located on chr1, region 11274211-11276515 REVERSE.

Physiological function[]

At1g31500.3/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g31500.3/Expression

Subcellular localization[]

At1g31500.3/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g31500.3 has 283 amino-acid residues and a molecular weight Mw = 32.172 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 5.88, or 0.021 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g31500.3/Knockout

Protein interactions[]

At1g31500.3/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g31500.3 has 1 recognized domains:

Domain_0058_0281 Exo_endo_phos domain (PF03372, IPR005135) Description: Endonuclease/exonuclease/phosphatase_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g31500.3 MFSSTTLHHL PRPNLLLPRK VISRRMSTNP AIEPKVRKFE SVEGVDIGSR NKSDGIRFRL VSYNILAQVY VKSALLPHSP PACLKWKARS HAILSVLKNL QADFFCLQEV DEYDSFYRNN MDSLGYSGIY IQRTGQRKRD GCAIFYKPSC AELVTKERIE YNDLVDSIKA DSVSCSEQKI ETSNEGKDSR KDSRDLNDPL VRLKRDCVGI MAAFRINKPF QHIVIVANTH LYWDPELADV KLAQAKYLLS RLAQFKTLIS DEFECTPSLL LAGDFNSIPG DMV ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-