In the plant Arabidopsis thaliana, At1g31540.1 is a gene model for the locus At1g31540.[1]
At1g31540.1/Header
Physiological function[]
At1g31540.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g31540.1/Expression
Subcellular localization[]
At1g31540.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At1g31540.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g31540.1/Knockout
Protein interactions[]
At1g31540.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At1g31540.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g31540.1
MASSSSSHNW
LYDVFLSFRG
EDVRVTFRSH
FLKELDRKLI
TAFRDNEIER
SHSLWPDLEQ
AIKESRIAVV
VFSKNYASSS
WCLNELLEIV
NCNDKIVIPV
FYHVDPSQVR
HQIGDFGKIF
ENTCKRQTDE
EVKNQWKKAL
TLVANMLGFD
SAKWNDEAKM
IEEIANDVLG
KLLLTTPKDS
EELVGIEDHI
AEMSLLLQLE
SKEVRMVGIS
GSSGIGKTTI
ARALFKRLSR
HFQGSTFIDR
AFVSYSRNIY
SGANPDDPNM
KLQLQGHFLS
EILGKKDIKI
DDPAALEERL
KHQKVLIIID
DLDDIMVLDT
LVGQTQWFGY
GSRIIVVTND
KHFLIAHGID
HIYEVSFPTD
VHACQMLCQS
AFKQNYAPKG
FEDLVVDVVR
HAGNFPLGLN
LLGKYLRRRD
MEYWMDMLPR
LENSLRIDGK
IEKILRISYD
GLESEDQEIF
RHIACLFNHM
EVTTIKSLLA
DSDVSFALEN
LADKSLIHVR
QGYVVMHRSL
QEMGRKIVRI
QSIDKPGERE
FLVDPNDIHD
ILNACTGTQK
VLGISLDIRN
IRELDVHERA
FKGMSNLRFL
EIKNFGLKED
GLHLPPSFDY
LPRTLKLLCW
SKFPMRCMPF
GFRPENLVKL
EMQYSKLHKL
WEGVAPLTCL
KEMDLHGSSN
LKVIPDLSEA
TNLEILNLKF
CESLVELPSS
IRNLNKLLNL
DMLNCKSLKI
LPTGFNLKSL
DRLNLYHCSK
LKTFPKFSTN
ISVLNLNLTN
IEDFPSNLHL
ENLVEFRISK
EESDEKQWEE
EKVSSSTLNI
ISKLFP
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.