In the plant Arabidopsis thaliana, At1g31740.1 is a gene model for the locus At1g31740.[1]
At1g31740.1/Header
Physiological function[]
At1g31740.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g31740.1/Expression
Subcellular localization[]
At1g31740.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At1g31740.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g31740.1/Knockout
Protein interactions[]
At1g31740.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At1g31740.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g31740.1
MSIYTIVLLF
LFHSRKRKFY
ITTMVSLSFI
LCCVLVSSCA
YATIVSHDGR
AITIDGHRRV
LLSGSIHYPR
STTEMWPDLI
KKGKEGSLDA
IETYVFWNAH
EPTRRQYDFS
GNLDLIRFLK
TIQNEGMYGV
LRIGPYVCAE
WNYGGFPVWL
HNMPGMEFRT
TNTAFMNEMQ
NFTTMIVEMV
KKEKLFASQG
GPIILAQIEN
EYGNVIGSYG
EAGKAYIQWC
ANMANSLDVG
VPWIMCQQDD
APQPMLNTCN
GYYCDNFSPN
NPNTPKMWTE
NWTGWYKNWG
GKDPHRTTED
VAFAVARFFQ
KEGTFQNYYM
YHGGTNFDRT
AGGPYITTTY
DYDAPLDEFG
NLNQPKYGHL
KQLHDVLHAM
EKTLTYGNIS
TVDFGNLVTA
TVYQTEEGSS
CFIGNVNETS
DAKINFQGTS
YDVPAWSVSI
LPDCKTETYN
TAKINTQTSV
MVKKANEAEN
EPSTLKWSWR
PENIDSVLLK
GKGESTMRQL
FDQKVVSNDE
SDYLWYMTTV
NLKEQDPVLG
KNMSLRINST
AHVLHAFVNG
QHIGNYRVEN
GKFHYVFEQD
AKFNPGANVI
TLLSITVGLP
NYGAFFENFS
AGITGPVFII
GRNGDETIVK
DLSTHKWSYK
TGLSGFENQL
FSSESPSTWS
APLGSEPVVV
DLLGLGKGTA
WINGNNIGRY
WPAFLSDIDG
DNTLVLFEEI
GGNPSLVNFQ
TIGVGSVCAN
VYEKNVLELS
CNGKPISAIK
FASFGNPGGD
CGSFEKGTCE
ASNNAAAILT
QECVGKEKCS
IDVSEDKFGA
AECGALAKRL
AVEAIC
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.