In the plant Arabidopsis thaliana, At1g31770.1 is a gene model for the locus At1g31770.[1]
At1g31770.1/Header
Physiological function[]
At1g31770.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g31770.1/Expression
Subcellular localization[]
At1g31770.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At1g31770.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g31770.1/Knockout
Protein interactions[]
At1g31770.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At1g31770.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g31770.1
MPQNCIAPRP
EEDGGVMVQG
LPDMSDTQSK
SVLAFPTITS
QPGLQMSMYP
ITLKFEEVVY
KVKIEQTSQC
MGSWKSKEKT
ILNGITGMVC
PGEFLAMLGP
SGSGKTTLLS
ALGGRLSKTF
SGKVMYNGQP
FSGCIKRRTG
FVAQDDVLYP
HLTVWETLFF
TALLRLPSSL
TRDEKAEHVD
RVIAELGLNR
CTNSMIGGPL
FRGISGGEKK
RVSIGQEMLI
NPSLLLLDEP
TSGLDSTTAH
RIVTTIKRLA
SGGRTVVTTI
HQPSSRIYHM
FDKVVLLSEG
SPIYYGAASS
AVEYFSSLGF
STSLTVNPAD
LLLDLANGIP
PDTQKETSEQ
EQKTVKETLV
SAYEKNISTK
LKAELCNAES
HSYEYTKAAA
KNLKSEQWCT
TWWYQFTVLL
QRGVRERRFE
SFNKLRIFQV
ISVAFLGGLL
WWHTPKSHIQ
DRTALLFFFS
VFWGFYPLYN
AVFTFPQEKR
MLIKERSSGM
YRLSSYFMAR
NVGDLPLELA
LPTAFVFIIY
WMGGLKPDPT
TFILSLLVVL
YSVLVAQGLG
LAFGALLMNI
KQATTLASVT
TLVFLIAGGY
YVQQIPPFIV
WLKYLSYSYY
CYKLLLGIQY
TDDDYYECSK
GVWCRVGDFP
AIKSMGLNNL
WIDVFVMGVM
LVGYRLMAYM
ALHRVKLR
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.