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In the plant Arabidopsis thaliana, At1g31800.1 is a gene model for the locus At1g31800.[1]

Also known as CYP97A3, and LUT5, this gene model is classified by TAIR as "CYP97A3/LUT5 (CYTOCHROME P450-TYPE MONOOXYGENASE 97A3); carotene beta-ring hydroxylase/ oxygen binding". It is located on chr1, region 11396421-11399451 FORWARD.

Physiological function[]

At1g31800.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g31800.1/Expression

Subcellular localization[]

At1g31800.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g31800.1 has 595 amino-acid residues and a molecular weight Mw = 66.838 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -6.44, or -0.011 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g31800.1/Knockout

Protein interactions[]

At1g31800.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g31800.1 has 1 recognized domains:

  • Domain_0111_0566 p450 domain (PF00067, IPR001128) Description: Cytochrome P450;Molecular Function: monooxygenase activity (GO:0004497), Molecular Function: iron ion binding (GO:0005506), Biological Process: electron transport (GO:0006118), Molecular Function: heme binding (GO:0020037)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g31800.1
MAMAFPLSYT PTITVKPVTY SRRSNFVVFS SSSNGRDPLE ENSVPNGVKS LEKLQEEKRR AELSARIASG AFTVRKSSFP STVKNGLSKI GIPSNVLDFM FDWTGSDQDY PKVPEAKGSI QAVRNEAFFI PLYELFLTYG GIFRLTFGPK SFLIVSDPSI AKHILKDNAK AYSKGILAEI LDFVMGKGLI PADGEIWRRR RRAIVPALHQ KYVAAMISLF GEASDRLCQK LDAAALKGEE VEMESLFSRL TLDIIGKAVF NYDFDSLTND TGVIEAVYTV LREAEDRSVS PIPVWDIPIW KDISPRQRKV ATSLKLINDT LDDLIATCKR MVEEEELQFH EEYMNERDPS ILHFLLASGD DVSSKQLRDD LMTMLIAGHE TSAAVLTWTF YLLTTEPSVV AKLQEEVDSV IGDRFPTIQD MKKLKYTTRV MNESLRLYPQ PPVLIRRSID NDILGEYPIK RGEDIFISVW NLHRSPLHWD DAEKFNPERW PLDGPNPNET NQNFSYLPFG GGPRKCIGDM FASFENVVAI AMLIRRFNFQ IAPGAPPVKM TTGATIHTTE GLKLTVTKRT KPLDIPSVPI LPMDTSRDEV SSALS
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment[]

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure[]

A predicted structure for this protein is available here.

References[]

  1. This is a test reference.

Further reading[]

General database links[]

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