At1g31930.1

In the plant Arabidopsis thaliana, At1g31930.1 is a gene model for the locus At1g31930.^[1]

At1g31930.1/Header

Physiological function[]

At1g31930.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g31930.1/Expression

Subcellular localization[]

At1g31930.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

At1g31930.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g31930.1/Knockout

Protein interactions[]

At1g31930.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

At1g31930.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g31930.1 MEKKDEGESW KEMVRKMLPP GAPLPEDPSE FDYSIALEYT GPPPVHDIPR VSPVDVNPRV NNPIPLPVSR IAGGVTSSSG GSPASSESVV SVLHNNPESS SGSASVSPVS GHRQNGNQVR RPVVKFKPVD DHDRIEGREA AEEEDNNVEA ETERERKVHE CTASTKRRKK KKKSECYRCG KAKWENKETC IVCDEKYCGN CVLRAMGSMP EGRKCVSCIG QAIDESKRSK LGKHSRVLSR LLSPLEVKQI MKAEKECTAN QLRPEQLIVN GYPLKPEEMA DLLNCLLPPQ KLKPGRYWYD KESGLWGKEG EKPDRVISSN LNFTGKLSPD ASNGNTEVYI NGREITKLEL RILKLANVQC PRDTHFWVYD DGRYEEEGQN NIRGNIWEKA STRFMCALFS LPVPQGQPRG TVQPSSNYAT VPNYIEHKKI QKLLLLGIEG SGTSTIFKQA KFLYGNKFSV EELQDIKLMV QSNMYRYLSI LLDGRERFEE EALSHTRGLN AVEGDSGGEE ANDEGTVTTP QSVYTLNPRL KHFSDWLLDI IATGDLDAFF PAATREYAPL VEEVWKDPAI QATYRRKDEL HFLPDVAEYF LSRAMEVSSN EYEPSERDIV YAEGVTQGNG LAFMEFSLSD HSPMSESYPE NPDALSSPQP KYQLIRVNAK GMNDSCKWVE MFEDVRAVIF CISLSDYDQI NITPESSGTV QYQNKMIQSK ELFESMVKHP CFKDTPFILI LNKYDQFEEK LNRAPLTSCD WFSDFCPVRT NNNVQSLAYQ AYFYVAMKFK LLYFSITGQK LFVWQARARD RANVDEGFKY VREVLKWDEE KEESYLNGGG EDSFYSTDMS SSPYRPEE ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-