At2g01480.1

In the plant Arabidopsis thaliana, At2g01480.1 is a gene model for the locus At2g01480.^[1]

This gene model is classified by TAIR as "similar to unknown protein [Arabidopsis thaliana] (TAIR:AT1G14970.1); similar to Hypothetical plant protein [Medicago truncatula] (GB:ABE89193.1); contains InterPro domain Protein of unknown function DUF246, plant; (InterPro:IPR004348)". It is located on chr2, region 216979-220340 FORWARD.

Physiological function[]

At2g01480.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At2g01480.1/Expression

Subcellular localization[]

At2g01480.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At2g01480.1 has 567 amino-acid residues and a molecular weight Mw = 64.576 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 27.53, or 0.049 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At2g01480.1/Knockout

Protein interactions[]

At2g01480.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At2g01480.1 has 1 recognized domains:

Domain_0159_0516 DUF246 domain (PF03138, IPR004348) Description: Protein of unknown function DUF246, plant_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At2g01480.1 MLAKNRLPGS GHTTPSPPAS PRRSPRYRHG RSKAAAGSRF PTVQPSRTLA HRLSWILLSV LLRRQGIFLF APLIYISCML LYMGTVSFDV VPIIQRRPPP GSVYKSPQVY AKLRPEMDAD NSTADAITTI WKHSYKGGEW KPYVNKSTGD LPESNGYIYV EANGGLNQQR TSICNAVAVA GYLNATLVIP NFHYHSIWRD PSKFGDIYDE EFFVSTLSND VRVVDTIPEY LMERFDHNMT NVYNFRVKAW SPIQYYRDSI LPKLLEEKII RISPFANRLS FDAPQAVQRL RCLANYEALK FSKTILTLGE TLVKRMKEQS ANHGAKYVSV HLRFEEDMVA FSCCIFDGGN QEKQDMIAAR ERGWKGKFTK PGRVIRPGAI RQNGKCPLTP LEVGLMLRGM GFNKSTYIFL ASGEIYDANR TMAPLLEMFP NLQTKEMLAS EEELAPYKNF SSRMAAIDYT VCLHSEVFVT TQGGNFPHFL MGHRRYMFGG HSKTIRPDKR KLAILFDNPN IGWRSFKRQM LNMRSHSDSK GFELKRPNDS IYTFPCPDCM SRRNKTTTPE SRPPPAT ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-