At2g01735.1

In the plant Arabidopsis thaliana, At2g01735.1 is a gene model for the locus At2g01735.^[1]

Also known as RIE1, this gene model is classified by TAIR as "RIE1 (RING-FINGER PROTEIN FOR EMBRYOGENESIS); zinc ion binding". It is located on chr2, region 324498-325894 FORWARD.

Physiological function[]

At2g01735.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At2g01735.1/Expression

Subcellular localization[]

At2g01735.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At2g01735.1 has 359 amino-acid residues and a molecular weight Mw = 40.089 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -9.79, or -0.027 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At2g01735.1/Knockout

Protein interactions[]

At2g01735.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At2g01735.1 has 1 recognized domains:

Domain_0307_0347 zf-C3HC4 domain (PF00097, IPR001841) Description: Zinc finger, RING-type;Molecular Function: protein binding (GO:0005515), Molecular Function: zinc ion binding (GO:0008270)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At2g01735.1 MSSYSSDSTA ARDQHAPLLR PRHDGSFSSS SSSARPTALA VLLGRITGHR APSMLVRETA ARALEERRID WGYSKPVVAA DILWNAALVL ASAVMLVGTV EERPNEPIRV WICVYGLQCL FHVVLVWSEY WRRNSTRRAR DLESYDHEDY NIEYDYEQDS DDNSTTYSFV KRCESINTVI SFIWWIIGFY WVVEGGDKLL GEAPNLYWLS VIFLAIDVFF AVFCVVLACL VGIALCCCLP CIIALLYAVA GTEGVSEAEL GVLPLYKFKA FHSNEKNITG PGKMVPIPIN GLCLATERTL LAEDADCCIC LSSYEDGAEL HALPCNHHFH STCIVKWLKM RATCPLCKYN ILKGTTDQS ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-