In the plant Arabidopsis thaliana, At2g26620.1 is a gene model for the locus At2g26620.[1]
At2g26620.1/Header
Physiological function[]
At2g26620.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At2g26620.1/Expression
Subcellular localization[]
At2g26620.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At2g26620.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At2g26620.1/Knockout
Protein interactions[]
At2g26620.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At2g26620.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At2g26620.1
MACIAFVLKA
LCLSLLLIVV
ASRSTIRPKV
FNVQRHGSKP
DGKTDNTNAF
TSIWSRACKR
ISGSSKIYVP
KGTFYLGGVE
FVGPCKNPIE
FIIDGTLLAP
ANPNDIKQDT
WINFRYINNL
SISGSGTLDG
QGKQSWPLND
CHKNPNCPKL
AISMGFAFVN
NSNIKDITSL
NSKMGHFNFF
FVHHFNITGV
TITAPSDSPN
TDGIKMGSCS
NIQISNTNIG
TGDDCIAILS
GTTKLNISNI
NCGPGHGISV
GSLGKNKDEK
DVKDLFVRDV
IFNGTSDGIR
IKTWESSASK
ILVSNFVYEN
IQMIDVGKPI
NIDQKYCPHP
PCEHERKSHV
QIQDLKLKNI
YGTSKNKVAV
NLQCSKSFPC
KNVELIDINI
KQNGLEDGSS
ITVCENVDGF
ARGKMFPQHC
LN
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.
