In the plant Arabidopsis thaliana, At4g10030.1 is a gene model for the locus At4g10030.[1]
This gene model is classified by TAIR as "hydrolase, alpha/beta fold family protein". It is located on chr4, region 6270487-6273095 REVERSE.
Physiological function[]
At4g10030.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At4g10030.1/Expression
Subcellular localization[]
At4g10030.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At4g10030.1 has 380 amino-acid residues and a molecular weight Mw = 42.155 kDa.
The protein is relatively basic; at the physiological pH 7.4, its net charge is 8.23, or 0.022 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At4g10030.1/Knockout
Protein interactions[]
At4g10030.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At4g10030.1 has 1 recognized domains:
- Domain_0136_0264 Abhydrolase_1 domain (PF00561, IPR000073) Description: Alpha/beta hydrolase fold-1_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At4g10030.1
MSAVSCSTAS
SSGGCAFRFR
SKTSIHSPNF
IRIGTNSQSD
RFCNHRLELV
HGTRKVKGAS
VRMALVEERQ
STSKDVAEPP
RVLAYDLVQG
ALVKWRWKDD
KSVPDTPTAV
LLHGILGSGK
NWGTFARRLA
HEFPTWQFLL
VDLRCHGDST
SLKKRGPHSV
ATTASDVLKL
VGQLRLTPRV
LVGHSFGGKV
VLSMVEQAAK
PLPRPVRAWV
LDATPGKVRA
GGDGEDHPRE
LISFLRKLPK
VVLSKREVLK
ALINEGFSND
VAQWVITNLR
PTGPSASSFS
WTFDLDGIAE
LYQSYEETNL
WNFVENLPRG
VHVNFLKAER
SLHRWALEDL
QRIHAAEELA
SEEGGGVEMH
VLEDAGHWVH
TDNPDGLFRI
LSSSFQVLRA
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.