In the plant Arabidopsis thaliana, At4g10465.1 is a gene model for the locus At4g10465.[1]
This gene model is classified by TAIR as "metal ion binding". It is located on chr4, region 6473578-6475133 FORWARD.
Physiological function[]
At4g10465.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At4g10465.1/Expression
Subcellular localization[]
At4g10465.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At4g10465.1 has 183 amino-acid residues and a molecular weight Mw = 21.025 kDa.
The protein is relatively basic; at the physiological pH 7.4, its net charge is 15.87, or 0.087 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At4g10465.1/Knockout
Protein interactions[]
At4g10465.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At4g10465.1 has 1 recognized domains:
- Domain_0053_0114 HMA domain (PF00403, IPR006121) Description: Heavy metal transport/detoxification protein;Biological Process: metal ion transport (GO:0030001), Molecular Function: metal ion binding (GO:0046872)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At4g10465.1
MSSLIVKSLG
SIVSIIARIF
FFRRSRPVSN
PRTTAHISYF
RMSRKRPLSL
QTVELKVRMC
CTGCVRIVRN
AISKLRGVDS
VEVDKELGRV
RVVGYVDRNK
VLKAVRRAGK
RAEFSPYPEP
PLYFTSTQNY
FVDPSKEFKE
SYNYYRHGYN
GTEQHGNIPV
GSRGDDRVSN
MFNDDNVNAC
RLM
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.